Abstract

Aim: Newcastle disease is an important viral disease for poultry caused by avian paramyxovirus which can be identified by its nature of agglutination activity with red blood cell of different species. The study was aimed to characterize the haemagglutinating activity of Newcastle Disease Virus at three different temperatures using RBC of five avian species, six mammalian species and eight different human blood group

Materials and Methods:
The study was carried out from January to December 2014 at Chittagong Veterinary and Animal Sciences University. Five avian and six different mammalian species were selected for the study. In each species two blood samples were collected aseptically. Eight different blood groups (A+, A-, B+, B-, AB+, AB-, O+, and O-) were studied in human. HA test were performed by using two virus strain ND Lasota and very virulent field isolate virus (VVNDV) with mentioned species of RBC at chilling (40c), incubating (370c) and room temperature (240c).

Results: Avian RBC require less time for agglutination than mammalian RBC. Incubation temperature (370c) require lowest time and chilling temperature require highest time for agglutination of RBC. Duck RBC require lowest time (17.81minutes) while chicken RBC needs highest (57.5 minutes) time for HA at incubation temperature and at chilling temperature respectively against ND Lasota virus and with field strain. Goat RBC require significantly higher time for HA (184.68 minutes) at chilling temp. than other mammalian species. Human RBC require almost similar time but O+ and O- blood group don’t show any HA activity. Significant variation (P

Key words: Key words: Newcastle disease virus, Haemagglutination, ND Lasota, Chilling temperature, Incubation temperature.