ABSTRACT
This study has investigated the effect of chemoterapy drugs combined CFderived from Lumbricus rubelus in HT-29 cells. HT-29 cells were treated with a combination of 5-fluorouracil (5-FU) (5 μg/ml) and Coleomic Fluid Lumbricus (CFL) (5 μg/ml, 10 μg/ml, or 20 μg/ml), respectively. The cells were incubated for 24 hours and then analyzed by flow cytometry to assess apoptosis, cell cycle progression, and proliferation. The cytotoxicity effect was measured by MTT assay that causes 50% decrease in cell viability (IC50 value). Results showed that the percentage of apoptosis in combined-therapy groups significantly higher than 5-FU single therapy group (p
Key words: Apoptosis, cell cycle, cell proliferation, cytotoxic effect, coelomic fluid, 5-FU
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