Objective: To evaluate the frequency of hospital and community-acquired MRSA in tertiary care hospital of Karachi, Pakistan, along with demographic factors and antibiotic susceptibility profile.
Methodology: This cross-sectional study was carried out in the Microbiology laboratory of Indus Hospital, Karachi, Pakistan. All specimens from patients of both gender and all ages from 2016-2020, except repeat isolates (sample from same patient within 30 days), were included in the study. The specimens were inoculated on appropriate media. Suspected Gram-positive colonies were confirmed on gram stain, followed by catalase test, coagulase and DNAse was performed for identification of S. aureus. Antibiotic susceptibility testing was performed by Kirby-Bauer technique by using Mueller–Hinton agar plates. Cefoxitin was used as surrogate antibiotic to rule out MRSA.
Results: A total of 4394 of S. aureus were isolated during study period from different clinical samples. Among these, 2741 were MRSA, 1471 were from males and 1270 were from females. The median age of the patients was found to be 29.36±20.11 years.
Conclusion: We observed high frequency of MRSA not only in the hospital but also in the community.
Key words: Community-acquired Infection, Frequency, Hospital-acquired Infection, Methicillin-resistant Staphylococcus aureus.
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