A study on the cytotoxic activity of Sungkai (Peronema canescens Jack) used in vitro cell lines has not been reported. Peronema canescens leaves contain many secondary metabolites with potential cytotoxic activity. In vitro cell lines assay can be used to determine natural products anticancer activity. This study aimed to identify secondary metabolites of P. canescens leaf chloroform fraction and subfraction and to determine the effect of cytotoxicity (IC50) on HT-29 colon cancer cells. In this study, the cytotoxicity activities on HT-29 cells were evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay using concentration ranges from 1.563 to 200.00 μg/ml. The detected secondary metabolites in the chloroform were alkaloids, terpenoids, steroids, flavonoids, and phenolics. The value of cytotoxic activity (IC50) against HT-29 colon cancer cells ranged from 14.807 to 34.448 μg/ml. The tests carried out showed that chloroform subfraction 3 showed potential cytotoxic activity in human HT-29 cancer cells with an IC50 value of 14.807 μg/ml and provided promising hope for further cytotoxicity studies.
Key words: Cytotoxicity, HT-29 cells, In vitro cell lines assay, Peronema canescens Jack, Secondary metabolites
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