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The development of multiplication conditions in vitro of Stevia rebaudiana Bertoni in Morocco

Bouaaza Ghizlane, Chetto Ouiam, Beniken Lhou, Dziri Laila, Benkirane Rachid, Hamid Benyahia.




Abstract

Stevia is an industrially and medicinally important plant used as a sweetener belonging to the family Asteraceae. The leaves of this plant contain a group of chemical compounds called steviol glycosides, which have been used as a natural sweetener substitute for sugar for a long time. The study aimed to develop a reproducible protocol for in vitro propagation of Stevia rebaudiana Bertoni. Nodal explants were cultivated on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP), Kinetin (Kin), and Indole-3-acetic acid individually or in combination for shoot induction. After a month, the leafy stems were placed in an MS medium supplemented with IAA and 1-Naphtalenacetic acid at 0.5 mg/L for rooting. MS medium without hormones (the control) produced the highest percentage of bud burst. The best treatment for shoots regeneration was MS medium supplemented with 1.5 mg/L BAP and 0.25 mg/L KIN. Medium with the highest multiplication rate was found to include MS fortified with 0.5 mg/L KIN. The medium MS supplemented with 0.5 mg/L IAA showed the maximum number of roots per shoot. About 93% of rooted plants were successfully acclimatized on pots containing peat (60%) and sand (40%).

Key words: micropropagation. Nodal explant. Self-incompatible. Stevia rebaudiana. seeds propagation, rooted plants.






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