The study aims to investigate effect of soaking Ammi visnaga seeds in the growth hormones gibberellic acid, kinetin, and naphthalene acetic acid (GA3, KN and NAA). Growth parameters at vegetative stage, in addition to the secondary compounds content especially khellin were measured using the HPLC analysis. Results showed that soaking in GA3 led to a noticeable increase in the length of the plant shoot, while the root depth increased by treatment with the NAA. Soaking seeds with GA3 100 ppm caused the highest plant weight with an increase by 63.38% compared with the control. High content of Chl. b and Carotenoids was observed by KN, while of Chl. it was by NAA. Soaking by the three plant hormones (GA3, KN and NAA) caused a slight increase in the content of the important medicinal component (khellin) in the plant seeds which suggested their utilization for increasing the medicinal value of Ammi visnaga.
Key words: Ammi visnaga, HPLC, khellin, growth hormones and Soaking.
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