Hyperlipidemia is a various group of disorders regarded as an excess of lipids in the blood stream, additionally it refers to increased concentrations of lipids (triglycerides, cholesterol, or both) in the blood. These lipids include cholesterol, cholesterol esters, phospholipids, and triglycerides. The current study aimed to investigate the role of green coffee beans extract in hyperlipidemic rats induced by poloxamer 407. Poloxamer-407 is a hydrophilic, non-toxic surfactant with low degree of toxicity and it have been stated that single intraperitoneal injection of Poloxamer-407 causes significant increase in serum cholesterol and triglyceride levels. Twenty four adult male albino rats were divided into four equal groups (each compromised of six rats); Group 1 was negative (normal) control, and the other 3 groups were made hyperlipidemic by intraperitoneal injection of 1.0 g/kg P-407. Group 2 was kept hyperlipidemic (HP) (positive control) and groups 3 and 4 were given GCBE orally by a gastric tube at a daily dose of 100 mg/kg (HP+GCBE100mg) and 400 mg/kg (HP+GCBE 400mg) respectively of body weight for four weeks. The obtained results revealed that induced hyperlipidemic caused significant increase in cholesterol, triglycerides, high density lipoprotein (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch), The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as compared with their relevant level in normal control rats group. Histological examination of liver tissue after P407 induction showed histological changes illustrated by the appearance of fatty change that was demonstrated by the accumulation of lipid in the form of large cytoplasmic vacuoles. A marked correction following treatments of HP rats with GCBE at dose 100 mg/kg or 400 mg/kg. The best amelioration occurred in the HP rats group which received 400mg of GCBE. The underlining mechanisms were discussed according to available references.
Key words: Green coffee beans extract, P-407, hyperlipidemic rats.
|
