Degradation of textile dyes using microbes has been recently identified as most promising, cost effective and eco-friendly technique than any physiochemical techniques. On this backdrop, a potential Reactive Black 5 dye degrading bacterium was isolated from effluent sediment samples of a textile industry, Salem district, Tamil Nadu, India which was identified as a Bacillus cereus SKB 12 based on morphological and biochemical methods. This bacterium exhibited promising dye degradation of Reactive Black 5 which was initiated from 1st hrs of lag phase and showed its maximum dye degradation of 88.7% at the end decline growth phase (144 hrs). Further, this strain revealed the synthesis of laccase, veratryl alcohol oxidase, lignin peroxidase, polyphenol oxidase and azoreductase enzymes with the presence of Reactive Black 5 dye. These overall observations proved that this strain, Bacillus cereus SKB 12 has the possibility to use as a potential textile dye effluent bioremediation agent.
Key words: Dye degradation; Reactive Black 5; Bacillus cereus; Screening; Growth kinetics; Dye degradation enzymes
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