Immunomodulators are compounds that are capable of enhancing the immune system. This research examined the immunomodulatory activity of Hibiscus sabdariffa L. flower fractions and identified the chemical compounds contained. A H. sabdariffa L. flower ethyl acetate extract was fractionated by the vacuum liquid chromatography method, yielding seven fractions: Fractions A, B, C, D, E, F, and G. Immunomodulatory activity testing was carried out by the phagocytosis method, and phytochemical analysis was conducted using liquid chromatography-mass spectroscopy. The test results showed that Fractions A, B, C, D, F, and G were significantly different from the negative control sodium carboxymethyl cellulose. Fraction A exhibited the best activity. The phytochemical analysis results showed that the H. sabdariffa L. flower fractions exhibited a number of compounds that have the potential to act as immunomodulatory active compounds.
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