Abstract
The study was undertaken to determine antibiotic resistance, ESBL production and transfer of resistant plasmids of Pseudomonas aeruginosa. Clinical samples of urine, wound and blood were processed and Pseudomonas aeruginosa identified following standard microbiological procedures. A total of 110 (18.3%) clinical isolates of Pseudomonas aeruginosa with higher prevalence in wound (43.6%) and blood (40.0%) were identified and included in this study. Identified isolates were profiled for antimicrobial susceptibility to a total of 10 antibiotics. Plasmid DNA extraction was carried out by the alkaline lysis method. ESBL production by phenotypic and genotypic methods were determined by double disc synergy test and by PCR techniques respectively. Transfer of resistant genes by conjugation using broth mating procedure was performed. High rates of resistance were observed in the antipsedomonal drugs tested. Seventy-seven (70.0%) of the isolates were multi drug resistance, out of which 26 (33.8%) and 4 (15.4%) produced ESBL by phenotypic and genotypic methods respectively. Plasmid profile analysis revealed that all the 77 MDR isolates harboured plasmids of >I.5kp. Four ESBL producing isolates selected could transfer their resistance plasmid to the recipient strain E. coli K-12. The therapeutic challenge observed calls for an effective antimicrobial stewardship program.
Key words: Multidrug-Resistance (MDR), ESBL production, Pseudomonas aeruginosa, antipseudomonal drugs
Key words: Multidrug-Resistance (MDR), ESBL production, pseudomonas aeruginosa.antipseudomonas drugs
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