Home|Journals|Articles by Year|Audio Abstracts

Original Article

Molecular identification of endophytic fungi associated with Coleus forskohlii (Willd.) Briq.

Grace Leena Crasta, Koteshwar Anandrao Raveesha.

Cited by 1 Articles

Coleus forskohlii (Willd.) Briq. is a notable medicinal plant belonging to the family Lamiaceae. Understanding the diversity of endophytic fungi associated with this medicinally important plant species and screening them to yield bioactive compounds would be highly useful for the herbal drug industry. The present study was undertaken to isolate and identify the diversity of fungal endophytes associated with C. forskohlii (Willd.) Briq. The fungal endophytes were isolated following standard procedures and molecular identification was carried out by using the 18S rRNA gene; the amplified regions were sequenced and submitted to NCBI, GenBank. A total of 85 endophytic fungi were isolated from 280 leaf segments. Molecular identification revealed 34 fungal genera. Among these, species of Cladosporium sp., Alternaria sp., Aspergillus niger, Aspergillus sp., Colletotrichum sp., Nigrospora oryzae, Penicillium sp., and Phyllosticta fallopiae were found to be predominant genera. The percentage occurrence of members of Ascomycota was the highest, with 96.47% distribution and Basidiomycota members were distributed the least, with 3.53%. The study revealed the diversity of endophytic fungi associated with the leaves of C. forskohlii and the phylogenetic tree shows the relationships between the endophytic fungi.

Key words: Coleus forskohlii (Willd.) Briq., Endophytic fungi, 18S rRNA sequence, Phylogenetic relations.

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.