The development of environmentally sustainable pathways for the synthesis of nanoparticals has been a major focus of nanotechnology research in recent years. The extracellular system for synthesis gold nanoparticles is used in this report in a greener, safer way by using local Proteus mirabilis. The addition of a hydrogen tetra chloro aurate (HAuCl4) solution to a Proteus mirabilis cell-free extract (CFE) resulted in the synthesis of gold nanoparticles (AuNPs) within 24 hours at room temperature (37oC). X-ray diffraction spectroscopy (XRD) analyses, Fourier Transform Infrared (FTIR) measurements, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy were used to characterize the nanoparticles. Proteus mirabilis can biosynthesize extracellular GNPs in the 28 nm range, according to our findings. Antibacterial activity of biosynthesized AuNPs against clinical MDR bacteria was discovered (Staphylcoccus aureus and Pseudomonas aeurogenosa), Also, The antibiofilm activity of AuNPs against the same microorganism used in the antibacterial activity has been investigated, the synthesized AuNPs were evaluated for their antioxidant activities (In vitro) of AuNPs from bacteria by DPPH assay.
Key words: Gold nanopartical, Antibacterial activity, Biofilm, Antioxident.
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