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Original Article

J App Pharm Sci. 2021; 11(12): 169-174


A new approach for evolution and quantification of Triamcinolone acetonide in medication shots by using RP-HPLC

Manikantha Naveen Vuddagiri, Veeraswami Boddu.


Abstract

Triamcinolone acetonide is a synthetic glucocorticosteroid has great immunosuppressive and anti-inflammatory activity. The most significant applications are to treat redness, itching, scaling, crusting like inflammatory skin disorders, and other conditions like dryness with psoriasis on the skin. The effective importance of Triamcinolone acetonide is the main objective of the study. A significant economic high responsive unique approach was developed for quantification of Triamcinolone acetonide in pharmaceutical dosage form by reverse phase high performance liquid chromatographic way. This drug samples were detached by passing through X-bridge phenyl column has a stationary phase and a mobile phase of 50:50 v/v of acetonitrile with octane sulphonic acid as a buffer solution to adjust the desirable pH-2.5 condition by employing ortho phosphoric acid. The Triamcinolone acetonide was eluted at 6.22 minutes with absorbance is 235 nano meters at input flow rate of 1ml/minutes with photodiode array detector. The linearity results of Triamcinolone acetonide gave regression equation y = 43,591 × + 14,344 and correlation coefficient is (R2) 0.9997. The percentage of relative standard deviation is 0.24 for intra-day precision and 0.65 for inter-day precision supports the ruggedness and the proposed approach is eco-friendly by AGREE-Analytical GREEnness metric shows 0.72. This new method was validated in accordance with the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines consisting of several analytical parameters like method precision, accuracy, ruggedness, robustness, and forced degradation studies results are supportive to this method for quantification and validation for Triamcinolone acetonide in medication shots and industrial use.

Key words: Glucocorticosteroid/ Triamcinolone acetonide/ Immunosuppressive/ RP-HPLC/ Forced degradation studies






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