Cellulose produced from microbial culture is known as bacterial cellulose (BC). BC has a high purity with no lignin, pectin and hemicellulose commonly found in plant cellulose and also high degree of crystallinity so that the microfiber contained in it is tightly bound together and forms very strong and elastic webbing. This study aims to provide a face mask sheet wherein the sheets are made from BC which is obtained from fermentation of coconut water using Acetobacter xylinum. The plant extracts used were green tea leaf extract (Camellia sinensis), roselle flower petals (Hibiscus sabdariffa), Hibiscus rosa-sinensis L. flower extract (red and pink varian of colour). The antioxidant assay using DPPH test for the BC with plant extracts was evaluated. The result showed that the highest antioxidant activity was owned by BC-green tea extract (IC50 80.9 ppm) followed by BC- red hibiscus extract (IC50 438,8ppm), BC-roselle extract (IC50 505,1ppm) and BC-pink hibiscus extract (IC50 1015ppm). Antibacterial testing was carried out using P. aeruginosa, S. aureus, E. coli, and S. typhi. The growth of bacteria incubated in BC extract with 37oC for 24 hours was carried out by optical density reading (OD) at 600 nm with ELISA microplate reader. The results obtained showed that with extract concentrations between 125 ppm-500 ppm; BC-rosela extract had the highest inhibitory activity against P. aeruginosa and E. coli. The BC-plant extracts were also characterized using SEM, FTIR and XRD for their morphological structure, functional groups and crystallinity. This result showed that bacterial cellulose with addition of plants extracts is a promising candidate for mask sheet application with natural antioxidant and antibacterial activity.
Key words: bacterial cellulose, antioxidant, antibacterial, plants extract, face mask sheet
|
