Probiotics are good microbiota which are able to promote the health of host immunomodulation. In this study, we prepared four antigens of Lactobacillus paracasei MSMC39-1 for the detection of tumor necrosis factor-α (TNF- α) secretion in hepatocellular carcinoma cell line (HepG-2). We found that viable cells and heat-killed cells of L. paracasei MSMC39-1 exhibited strong TNF-α secretion inhibitors. Viable cells of L. paracasei MSMC39-1 treatment significantly reduced serum level of aspartate aminotransferase in hepatitis rats. TNF-α secretion in liver tissues of hepatitis rats in probiotic L. paracasei MSMC39-1 treatment was significantly suppressed. Liver histopathology examination of alcohol-induced hepatitis indicated fat accumulation in the hepatocytes. Interestingly, liver damage in hepatitis rats was improved by probiotic L. paracasei MSMC39-1. In addition, L. paracasei MSMC39-1 alleviated colon inflammation in alcohol-induced hepatitis rats. Administration of L. paracasei MSMC39-1 modulated gut microbiota by increasing the number of genera Lactobacillus and Bifidobacterium in alcohol-induced hepatitis rats. We conclude that L. paracasei MSMC39-1 has potent anti-inflammatory effects on HepG-2 cell line and improves hepatitis, colon inflammation, and modulates gut microbiota. Thus L. paracasei MSMC39-1 is able to protect against alcoholic hepatitis. It can also be used as an alternative treatment in other inflammation diseases and applied in nutraceutical and dietary supplement products.
Key words: Probiotics, Hepatocellular carcinoma cell line, alcoholic hepatitis, Tumor necrosis factor, Lactobacillus paracasei
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