Mimosa pudica is used in traditional medicine to help with diabetes therapy. This study was conducted to analyze phytochemicals and evaluate the protective effect of ethanol M. pudica Linn. extract on glucotoxicity induced by methylglyoxal (MGO). Mimosa pudica leaves were extracted with 80% ethanol at room temperature using the cold maceration method, repeated three times. Liquid chromatography-mass spectrometry (LC-MS) technique was used to investigate the phytochemicals in the ethanol sample. The protective effects of M. pudica ethanol extract on glucotoxicity in human umbilical vein endothelial cells (HUVECs) inhibit advanced glycation end product (AGE) formation and also the breaking effects of M. pudica ethanol extracts on MGO and glyoxal- (GO-) AGEs were measured. Our results showed that M. pudica extract protects against MGO-induced glucotoxicity, prevents MGO-induced AGE production, and eliminates preformed AGEs. We have identified several chemical components of M. pudica by -LC-MS, including 2-tert-butyl-2-phenyl-1,3-dioxolane, 4-(2-phenylethyl)-phenol, 4-phenylbutan-2-ol, 1-naphthalenecarboxylic acid, ferulic acid, myoinositol, caffeic acid, tyrosinamide, 2-hydroxy-benzene ethanol, p-hydroxybenzoic acid, luteolin, fisetin, apigenin, gallic acid, quercetin, monoamidomalonic acid, jasmonic acid, 3-fluoro-p-anisidine, and naringenin. According to our findings, M. pudica extract has a protective effect on suppressing GO-induced glucotoxicity in HUVECs.
Key words: Mimosa pudica Linn.; Advanced glycation end products (AGEs); Methylglyoxal (MGO); Glyoxal (GO); Human umbilical vein endothelial cells;
|
