Clostridium perfringens (C.perfringens) induced necrotic enteritis (NE) has become a worldwide significant problem in broilers industry. A total of 100 intestinal samples were collected from broilers chickens that demonstrated clinical manifestations and post mortem lesions of Necrotic Enteritis. Bacteriological examination of samples revealed that C. perfringens was isolated from (62/100) 62%. PCR was used to screen the circulating toxigenic subtypes and revealed that 14/20 (70%) of isolates were positive to cpa gene thus belonged to C. perfringens type A. Phenotypic antimicrobial resistance patterns was demonstrated by isolates to lincomycin 51/62(82.26%), ampicillin 45/62 (72.6%), nalidixic acid 43/62(69.35%), and spectinomycin 43/62 (69.35%), respectively. Futhermore, 49/62 (79%) of isolates demonstrated multidrug resistance phenotypes. Experimental study demonstrated better performance indicators in C.perfringens challenged chicks fed on probiotic (Bacillus subtilis) and prebiotic (Mannan Oligosaccharide / MOS) compared to the challenged chicks treated with amoxicillin.
In conclusion, the prevalence of multidrug resistant C.perfringens in infected broilers flocks, signified the need for applying AMR surveillance and raising awareness on the proper use of antibiotics in broilers sector. The promising results of NE prevention associated with feed supplementation by Bacillus subtilis and Mannan Oligosaccharide (MOS) represented an effective alternative to be used in conjunction with good management for prevention of NE rather than its control by antibiotics.
Key words: Bacillus subtilis, Clostridium perfringens, Mannan Oligosaccharides (MOS), Multidrug resistance, PCR.
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