Oxyresveratrol is a polyphenolic compound found in Mulberry (Morus albaL.) twigs and has known as a skin lightening agent. Many methods can be applied to extract oxyresveratrol from Mulberry twigs. This research aimed to optimize the extraction method by using surfactant Tween 80 and Tween 20-based microwave-assisted extraction (MAE). Extraction parameters, including solvent concentration, liquid-solid ratio, and extraction time for oxyresveratrol, were optimized using response surface methodology (RSM) based on Box-Behnken Design (BBD). This research also extracted the oxyresveratrol by maceration, then the oxyresveratrol content from each extraction methods were compared. Oxyresveratrolcontent was determined using High-Performance Liquid Chromatography (HPLC). For Tween 80, the optimum condition was obtained at 10.5 mM, 30:1 ml/g liquid-solid ratio, and 10 min extraction time. For Tween 20, the optimum condition was obtained at 100 mM, 40:1 ml/g liquid-solid ratio, and 5 min extraction time. Oxyresveratrol content was 0.0146 mg/g dried sample, and 0.0172 mg/g dried sample at the optimum condition of Tween 80 and Tween 20 surfactant, respectively. Meanwhile, the oxyresveratrol content of the maceration method with 96% ethanol was 1.5704 mg/g dried sample. In conclusion, these results show that the application of Tween 80 and Tween 20 as solvents for MAEof Oxyresveratrol from mulberry twigs were not fully successful since other extraction conditions should be considered, such as temperature, pH, and microwave energy.
Key words: microwave-assisted extraction (MAE), Morus alba, oxyresveratrol, surfactant, Tween 20, Tween 80
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