Abstract

The emerging resistance to anti-fungal agents encourage the exploration of new and effective natural product showing anti-fungal activity to significantly impact the treatment and the management of biofilm associated fungal infections. In nature, most of microbes live in form of biofilms, and up to 80% of microbial infections on human body are biofilms associated. This research aimed at finding out antibiofilm activity of Bajakah tampala extract against Candida albicans. The inhibition and eradication activities toward C. albicans biofilm were tested using microtiter broth method by measuring the minimum value of biofilm inhibition concentration (MBIC50) and minimum biofilm value eradication concentration (MBEC50). The C. albicans biofilm structure in front of and absence of the extracts was analysed using SEM. At concentration of 1% w/v Bajakah tampala extract showed activity against C. albicans mid-phase biofilm formation and maturation phase (77.00% ± 0.01, 70.31% ± 0.01) and breakdown the established biofilms as much as 65.21% ± 0.01. The biofilm architecture analysis by SEM provided evidence that Bajakah tampala ethanol extract disturbed the extracellular polymeric substance (EPS) matrix of the C. albicans biofilm. The result obtained clearly indicate that Bajakah tampala exthanol extract can developed as a new antibiofilm candidate for the treatment of C. albicans biofilm infection.

Key words: Bajakah tampala, biofilm, C. albicans, infection, antibiofilm