Aim: The study determine and evaluate the prevalence and antibiotic resistance of Staphylococcus aureus (S. aureus) isolated from milk collected along the milk value chain from farm herds, milk collection centre and milk shops in Mbeya rural and Mbozi districts, Tanzania.
Materials and Methods: A total of 150 milk samples were collected; 96 from farmers herds, 18 from milk collection centers and 36 from milk shops. The samples were cultured in Manitol salt agar for pathogen isolation and biochemical tests performed for confirmation of S. aureus. Kirby-Bauer disc diffusion method was employed for antibiotic resistance testing.
Results: One hundred and forty samples yielded Staphylococcus species; these were from farmers herd (92), milk collection center (18) and milk shops (30), respectively. Biochemical tests showed that 21 (15%) were positive for S. aureus. The corresponding prevalence rates from the value chain nodes were 14.1%, 16.7% and 16.7%, respectively. Resistance to Penicillin was frequently observed (57.1%) and Vancomycin was sensitive to all S.aureus isolates tested. Resistance along the sampling points showed significant positive correlation (r=0.82, p
Key words: Milk products, multiple antibiotic resistant, milk contamination
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