Home|Journals|Articles by Year|Audio Abstracts

Original Article

Metabolic fingerprinting of Melastoma malabathricum L. extracts using high-performance liquid chromatography-diode array detector combined with chemometric data analysis

Dian Mayasari, Yosi Bayu Murti, Sylvia Utami Tunjung Pratiwi, Sudarsono Sudarsono.

Cited by 2 Articles

Melastoma malabathricum L. is an essential herb that has been traditionally used to treat several ailments such as diarrhea, dysentery, wound, and stomachache. This plant is common across tropical Indo-Pacific archipelagos distributed in various geographical locations of Indonesia. In this study, an effective, accurate, and efficient highperformance liquid chromatography (HPLC) coupled to diode array detectors was developed to evaluate the effect of geographical variations on secondary metabolites of M. malabathricum samples through establishing a fingerprint profile, identification of variations of chromatogram peaks, and determination of anti-methicillin-resistant Staphylococcus aureus (MRSA) activity. Fingerprinting analysis of the extracts was analyzed using chemometric tools including unsupervised data analysis: principal component analysis (PCA) and hierarchical cluster analysis (HCA). PCA values were identified as principal component 1 (PC1) and principal component 2 with 41.229% and 19.208%, respectively. Anti-MRSA activity of all samples showed significant inhibition against MRSA bacteria. The results indicate the significance of considering geographic distribution during field-collection efforts since they demonstrate regional metabolic variations in secondary metabolites of M. malabathricum L., as illustrated by HPLC and their anti-MRSA activity. The results also confirm the utility of this approach as a comprehensive evaluation of metabolic variations based on their different geographical locations and pharmacological effects.

Key words: anti MRSA, chemometricts, HCA, HPLC, Melastoma malabathricum L., PCA

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.