Breast milk is a source of lactic acid bacteria (LAB) with remarkable functional properties and has a potential therapeutic role on gastrointestinal disease. It is also a potential source of probiotics. The objective of this study was to identify genotypic as well as phenotypic characterization of LAB isolated from human milk. The LAB strains were tested and proved to inhibit the growth of Vibrio cholerae (ATCC 14035). The isolates also exhibited bile salt tolerance and antioxidant activity. Among the seven isolates, PB7 proved to be the best potent probiotic. Thus, the optimum pH and temperature of PB7 were determined. The morphological characterization of PB7 was confirmed as rods through the scanning electron microscope. The selected isolate, PB7, was then subjected to 16S ribosomal ribonucleic acid sequencing and obtained an accession no. MN121704 in National Centre for Biotechnology Information, which was named as Lactobacillus fermentum (BURD PB7). The combination of this probiotic (L. fermentum) and prebiotic (Vitamin C) is known as synbiotic. When this synbiotic was applied to the colitis-induced mice model, the score of the Disease Activation Index showed that it was a better therapeutic tool in comparison to the probiotic.
Key words: Keywords: Breast milk, Disease Activation Index, Colitis, Lactobacillus fermentum, Probiotic, Prebiotic, Synbiotic.
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