Abstract

Aim: This study aimed to investigate the effect of amniotic membrane wrapped onto the anastomosis line following colon resection and primary anastomosis on anastomosis healing in rats undergoing pelvic radiotherapy.
Material and Methods: Fifty-six male Wistar albino rats were randomly allocated to four main groups. Group I, left colon resection and primary anastomosis; Group II, post-anastomotic amniotic membrane; Group III, preoperative radiotherapy; Group IV, preoperative radiotherapy and amniotic membrane. Radiotherapy was administered under general anesthesia eight and four days prior to surgery at a total of 20 Gy. In the first operation, all the rats underwent 1 cm left colon resection and primary anastomosis. The animals in each group were anesthetized to assess the clinical, mechanical, histologic, and biochemical parameters of anastomotic healing on the third and seventh postoperative days.
Results: In the third day groups, anastomosis line bursting was observed in only one subject in those undergoing amniotic membrane wrapping(p

Key words: Radiotherapy; colorectal anastomosis; anastomosis healing; amniotic membrane