Abstract:
Flemingia tuberosa Dalzell is an endemic species of Western Ghats. It is an ethnomedicinal plant used to cure dysentery and vaginal discharge. However in the current study the hepatoprotective potency of the plant has been evaluated as it contains flavonoids like kaempferol. The present study was conducted to investigate the hepatoprotective activity of the ethanolic extract of aerial parts of Flemingia tuberosa against CCl4-induced to toxicity in rats. The extract was standardized using validated HPTLC method for kaempferol content using HPTLC. The antioxidant activity of ethanolic extract of aerial parts of Flemingia tuberosa was assessed by DPPH method. Hepatoprotective activity was assessed against CCl4 induced liver intoxication wherein the CCl4 induced albino Wistar rats were treated with three different doses of the standardized extract for three days. The results were compared with the established positive control, Silymarin. Protective effect on liver was also monitored histopathologically using light and electron microscope and biochemically through observing the levels of biochemical enzymes. The extract showed potent Hepatoprotective activity. This study confirms the protective effect of ethanolic extract of aerial parts of Flemingia tuberosa against CCl4-induced hepatotoxicity.
Key words: Flemingia, endemic, chromatography, antioxidant, hepatoprotective, histopathology
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