The present study was conducted to determine the heavy metal accumulation concentrations in different tissues of the selected brackish water fish Mugil cephalus. The fishes were exposed to sublethal concentrations of lead acetate (Pb (C2H3O2)2) for 96 hr. and LC50 value was found to be 18.7ppm. The one-tenth of (1/10) LC50 (1.89 ppm) value was selected as lower sub-lethal concentration for the exposure period of 30 days. The organs of fish viz., gills, liver, and muscle were carefully separated through dissection from fish for determination of heavy metal using Absorption Spectrophotometer (Perkin Elmer Analyst-400, USA). The results reveal that the low concentration of lead (1.89 ppm) exhibits a maximum level of accumulation in the liver (0.746 ± 0.0033 μg/g), gills (0.672±0.0069μg/g) and muscle (0.254±0.0052 μg/g) tissues of 30 days exposed. The result of the present investigation also reveals that the time-dependent lead accumulation in the different tissues of fish Mugil cephalus exposed to different sub-lethal concentration of the lead. These findings extend for future studies on the evaluation of lead accumulation tendency in relation to the eco-toxicological observations for heavy metals risk assessment.
Key words: Bioaccumulation, Heavy metal, Lead (Pb) toxicity, LC50 lethal concentrations, brackish water fish, Mugil cephalus.
|