Desulfovibrio spp. is the main cause of hydrogen sulfide H2S gas and organic pollution in shrimp ponds. The amount of food deposited at the pond bottom provides favourable conditions for the growth of H2S gas-producing bacteria. Many studies have shown that the use of antibiotics to treat bacteria led to the emergence of multidrug-resistant strains, which has raised awareness of the critical need for alternative antimicrobials. Bacteriophages – viruses that infect and inhibit bacterial cells – are currently of great interest as highly viable alternatives to antibiotics. The purpose of this study is to evaluate the effectiveness of bacteriophage in controlling H2S-producing bacteria in shrimp ponds. The bacterial strains were identified based on morphological, physiological, and biochemical criteria. The bacterial species were molecularly identified using the 16S rRNA gene sequencing, and phage strains capable of inhibiting H2S gas-producing bacteria were investigated by agar spot test and plating method. The result investigated six strains of bacteria, Desulfovibrio spp., that were isolated from the shrimp pond. This study showed that phages L12 and T0 could infect bacteria Desulfovibrio spp. and prevent the growth of the bacteria 11D as well as prevent the generation of H2S gas.
Key words: Bacteriophages, Black colonies, Desulfovibrio spp., H2S gas-producing bacteria, Organic pollution, 16S rRNA gene sequence.
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