Fipronil (FPN) is a broad-spectrum insecticide widely used in agriculture and veterinary applications. FPN repressed the cellular enzymatic and non-enzymatic antioxidants, resulted in oxidative injuries. Both nigella sativa oil (NSO) and resveratrol (RSV) had antioxidant activities. The present study aimed to assess the ameliorative effect of NSO and RSV against FPN induced toxicosis in male albino rats. Rats were divided into six groups. The first group was administered FPN (10 mg/kg); the second received FPN (10 mg/kg) and NSO (1ml/kg); the third group received FPN (10mg/kg) and RSV (20mg/kg); fourth, and fifth were treated with NSO (1ml/kg) and RSV (20mg/kg), respectively. The sixth group was the control. The results showed that FPN significantly increased serum levels of urea, creatinine, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. FPN-intoxicated rats revealed a significant increase in the level of MDA and significant decrease in GSH level in liver and brain tissues. While, kidney tissue revealed a significant decrease in renal GSH concentration and non-significant increase in renal MDA. Furthermore, FPN showed histological changes in liver, kidney, brain and heart tissues. In contrast, administration of NSO and RSV ameliorated the FPN-induced oxidative damage, and histopathological alterations possibly due to their antioxidant properties.
Key words: Fipronil, nigella sativa, resveratrol, histopathology
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