Most of the researchers have been reported about the nontoxic biosynthesis of silver nanoparticles using several microorganism and plant
extracts. This ecofriendly silver nanoparticles exhibit completely new or improved properties based on specific characteristics such as size,
distribution, morphology and many applications. In this research the synthesis of silver nanoparticles using Piper betle has been
investigated. We have synthesized silver nano particles using 1 mM silver nitrate solution into the plant extract and characterized by UVvis absorption spectroscopy. The antibacterial efficacy also determined by disc diffusion method with Bacillus cereus, Escherchia coli,
Klebsiella pneumonia, Staphylococcus aureus and it showed that high level of inhibition. The most outcome of this research will be
suggested that biologically synthesized silver nanoparticle has more effective against various disease causing pathogens.
Key words: Piper betle, Silver nanoparticles, antibacterial activity
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