Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

Optimization of ionic liquid-based microwave-assisted extraction on brazilin levels from sappan wood and its dipeptidyl peptidase IV inhibition activity

Islamudin Ahmad, Erwi Putri Setyaningsih, Ayun Erwina Arifianti, Fadlina Chany Saputri, Abdul Mun’im.

To elevate brazilin levels from sappan wood (Caesalpinia sappan) using the optimized ionic liquid-based microwave-assisted extraction (IL-MAE) method and to evaluate its dipeptidyl peptidase IV (DPP-IV) inhibition activity. According to the preoptimization results, the IL-MAE method condition’s optimization was carried out using response surface methodology with the Box–Behnken design. Brazilin levels were determined by the high performance liquid chromatography (HPLC) gradient method (0.3% acetic acid in water and acetonitrile). A DPP-IV inhibition activity assay was then conducted using the DPP-IV-GloTM kit and determined using Glomax (Promega, Madison, WI) with an excitation of 350–360 nm and an emission of 450–465 nm. The most optimized extraction condition was 1.5 mol/l 1-butyl-3-methyl imidazolium bromide ([BMIM]Br), 1:20 g/ml solid-to-liquid ratio, and 9 minutes of extraction time. HPLC analysis of sappan extract showed brazilin levels of 807.56–948.12 mg/g extract. Sappan extract gives the in vitro inhibitory value of DPP-IV activity of >90%. The inhibition concentration of 50% value was obtained including 9.37 µg/ml (sitagliptin), 11.7 µg/ml (brazilin standard), 16.35 µg/ml (extract sample), and 22.15 µg/ml (tannin removal sample). In the present study, imidazolium basic IL-MAE was optimized and first applied to elevate the brazilin levels from sappan wood. Thus, this is an optimum extraction condition for elevating sappan wood’s brazilin levels rapidly, efficiently, quickly, and environmentally friendly.

Key words: Brazilin, Dipeptidyl peptidase-IV, ionic liquid-based microwave-assisted extraction, response surface methodology, sappan wood (Caesalpinia sappan)

Similar Articles

A Transfer Learning-Based Approach with Deep CNN for COVID-19- and Pneumonia-Affected Chest X-ray Image Classification.
Chakraborty S, Paul S, Hasan KMA
SN computer science. 2022; 3(1): 17

Multiclass cyanotoxin analysis in reservoir waters: Tandem solid-phase extraction followed by zwitterionic hydrophilic interaction liquid chromatography-mass spectrometry.
Aparicio-Muriana MM, Carmona-Molero R, Lara FJ, García-Campaña AM, Del Olmo-Iruela M
Talanta. 2022; 237(): 122929

SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles.
Aithal S, Mishriki S, Gupta R, Sahu RP, Botos G, Tanvir S, Hanson RW, Puri IK
Talanta. 2022; 236(): 122841

Does the Covid-19 pandemic affect faith-based investments? Evidence from global sectoral indices.
Dharani M, Hassan MK, Rabbani MR, Huq T
Research in international business and finance. 2022; 59(): 101537

Efficient production of oligomeric chitin with narrow distributions of degree of polymerization using sonication-assisted phosphoric acid hydrolysis.
Zhang X, Mao Y, Briber RM
Carbohydrate polymers. 2022; 276(): 118736

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.

ScopeMed Web Sites