Background and Aim: The problem of antimicrobial resistance bacteria in both humans and animals is an important public health concern for worldwide wich is more likely to increase including in Thailand especially Carbapenem-Resistant Enterobacteriaceae (CRE), such as E. coli. It is a pathogen in the gastrointestinal tract that can be found in all human, animals and the environment as well as an opportunistic infection and often resist to antibiotic in various fields especially in animal husbandry, such as pets or livestock farms. This study aims to investigate the occurrence of Carbapenem - Resistant E. coli from water samples in Thai dairy farms.
Materials and Methods: Water samples were collected from 32 dairy farms in Kaeng Khoi district, Muak Lek district and Wang Muang District of Saraburi Province, including Kantharawichai district and Mueang district of Maha Sarakham province, Thailand. All samples were cultured and isolated for E.coli by biochemical tests. All E.coli isolates were tested for drug susceptibility by using imipenem, meropenem and detect drug resistance genes of Carbapenemeses such as blaNDM, blaIMP and blaOXA48 of drug resistant E.coli isolates by PCR technique.
Results: The results found that total E. coli isolation were 182 isolates (140 isolates from Saraburi and 42 isolates from Maha Sarakham, respectively) and after drug sensitivity test found that 2 isolates of E. coli resistant to imipenem which were derived from water in Kaeng Khoi district. It can be considered the incidence of E. coli resistant to carbapenem as 1.43% of Saraburi province. In the other hand, there was no incidence of drug resistance E. coli in Maha Sarakham. In addition, the detection of drug resistant gene of E. coli in both isolates by PCR showed the expression of blaNDM.
Conclusion: This study point out the situation of E. coli which resist to antimicrobial drug in livestock farms. Moreover, it might be considered to be the first report of E. coli CRE detection in a dairy farm at Saraburi which should be further extended study.
Key words: water, antimicrobial resistance, E. coli, dairy farm, CRE
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