The present study was conducted on the protein extracted from the pulp that obtained by cannabis oil-pressing and loading the bioactive peptides into nanoliposomes. Physical properties of empty and loaded nanoliposemes (average particle size, PDI, Zeta potential) and encapsulation efficiency was evaluated. Moreover, the effect of storage condition (fridge and ambient temperature) on the physical stability and maintaining the encapsulation efficiency in nanoliposomes loaded with peptides was investigated. Eventually, the effect of peptide loading on the nanoparticle chemical structure (FTIR) as well as the morphology of nanocarriers (SEM) was evaluated. Physical properties of nanoliposomes were affected by the hydrolyzed type. The average particle size and polydispersity index of nanoliposomes varied from 79.5-101.5 nm and 0.234-.326, depending on the type of loaded peptides. The zeta potential of nanoliposomes was changed from -10.32 to -15.33 mV when loaded with the peptide obtained by enzymatic hydrolysis during 300 minutes. The efficiency of nanoliposomal encapsulation varied from 90.7 to 81.4 percent. Thus, peptides with the highest degree of hydrolysis had the least encapsulation efficiency. The evaluation of physical stability and the maintenance of encapsulation efficiency indicated the highest stability of nanoliposomes, which were stored at fridge temperature. FTIR spectroscopy in empty nanoliposomes and those loaded with peptides implied the presence of peptides in the polar regions of phosphatidylcholine, such as the internal regions of the liposomes and the formation of ionic complexes between them. Conversely, scanning electron microscopy (SEM) images of the structural and superficial properties of nanoliposomes indicated the existence of dense and compact clusters of the spherical nanoparticles with flat surfaces.
Key words: Nanoliposome, Bioactive Peptide, Enzymatic Hydrolysis, Cannabis
|
