Abstract
This study was conducted to investigate the effects of tributylin chloride (TBTCl) on epithelial cell of gut Artemia salina in different stages (Nauplii, Juveniles and Adults). Samples of A. salina used were cultivated in incubators for hatching. Nauplii were harvested at 24 hours of age, while the juveniles and adults were harvested at 21 and 35 days of age respectively. These three stages of A. salina were exposed to different concentrations of TBTCl (1 ng.L-1 to 500 ng.L-1) for 24 hours. For nauplii, juveniles and adults, 100 individuals were exposed, and those that survived in the exposure test were harvested for histological analysis. Results of histological examinations revealed significant differences (P < 0.05) in type of lesions associated with different TBTCl concentrations and at different stages. The predominant lesions associated with different stages and different concentrations of TBTCl were epithelial cell necroasis, degeneration, cell loss, disruption, piknosis and submucosal necrosis. Cell scoring was significant difference (P < 0.05) between the groups of different TBTCl concentration and different life stages. The generality of the lesion scores showed that the adults are relatively more susceptible to the effects of TBTCl compared to the juvenile and the nauplii.
Key words: Antifouling biocides; Artemia salina; Histology; Tributyltin chloride; Toxicity.
|
