In this study, the interaction of hesperidin with -CD, HP--CD, -CD sulfate was investigated by thin layer chromatography. The
chromatographic conditions included: Kieselgel G25 stationary phase, mobile phase of butanol: acetic acid: water (4:1:5) and increasing
concentrations of the three cyclodextrins. Visualization was performed by examination in UV light and by exposure to iodine vapors. Also,
the thermodynamic parameters were calculated: the Gibbs free energy change (G0
tr), free energy change (ΔG0
), enthalpy change (ΔH0
)
and entropy change (ΔS
0
). The results show that the best inclusion capacity is obtained for HP--CD, the inclusion process is favored by
increasing the concentration of cyclodextrin, and by increasing the temperature.
Key words: : hesperidin, cyclodextrin, thin layer chromatography, thermodynamics
|
