Abstract

Objective: To observe the protective effects of turmeric against methotrexate induced hepatotoxicity.
Methodology: This experimental study included 28 adult male albino mice which were divided into control Group A and experimental Groups, B, C and D. In control Group A, 4 mice, and in Group B, C and D 8 mice were included. To group A, no drug was given. Turmeric extract per oral (400mg /kg) daily was given to Group B for 14 days. Injection methotrexate (40mg/kg) intraperitoneal (I.P) was given to Group C on day 7. Turmeric extract per oral (400mg /kg) daily for 7 days was given to Group D as pre administration to injection methotrexate and on day 7 injection methotrexate (40mg/kg) was given intraperitoneally (I.P) and turmeric extract per oral (400mg/kg) was given for further 7 days daily, post administration to injection methotrexate. All the animals were dissected on day 14 and liver was examined under light microscope to study histological parameters.
Results: The histological examination of liver sections of group A (control) showed normal appearance of portal vein. In Group B (turmeric treated group), no obvious histological changes were observed. Examination of liver tissue of Group C (methotrexate group) showed severe histological changes, which included congestion of portal vein. Group D (methotrexate +turmeric group) showed significant reduction in congestion of portal vein.
Conclusion: This study clearly demonstrates that administration of turmeric (Curcuma Longa) protects liver tissue.

Key words: Turmeric, methotrexate, liver damage, congestion in portal vein, antioxidant.