Flax is an oilseed and medicinal plant that numerous breeding lines and cultivars have produced by plant breeders up to now. The present study was conducted to evaluate the biochemical factors affecting by NaCl salt stress in salt susceptible and tolerant flax genotypes. The experiment was arranged as factorial based on a completely randomized design with three replications in greenhouse conditions. The experimental factors included Tabare, Golchin and 375Ha genotypes as the first experimental factor and salinity stress (120 mM NaCl) time course at three levels (0, 24 and 48 hours after salinity treatment) as the second factor. The measured biochemical factors in the leaf were catalase, guaiacol peroxidase, malondialdehyde, hydrogen peroxide, proline and superoxide dismutase. The results showed that salinity stress had a significant effect on the content of measured biochemical parameters. The levels of catalase, guaiacol peroxidase, hydrogen peroxide and proline were statistically significant in the flax genotypes. Furthermore, the interaction of genotype and time after applying stress had a significant effect on the content of catalase, hydrogen peroxide and proline. The amount of catalase and proline in the 24 hours after stress was more than the 48 hours, indicating the key role of these factors in the beginning of the stress. On the other hand, the amount of guaiacol peroxidase and superoxide dismutase increased significantly in 48 hours after stress. Generally, the content of antioxidant factor increased significantly under salinity stress, especially in 375Ha tolerant genotype. This indicates the importance of these enzymes in salt stress conditions in order to more accurately evaluate the genotypes sensitive and tolerant to the flaxseed in seedling stage.
Key words: Antioxidant Enzymes, Catalase, Malondialdehyde, Proline, NaCl Stress, Flax
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