AIM: The aim of the study is to find the antidiabetic activity of piper longum and stevia herbal formulation.
INTRODUCTION: Diabetes Mellitus also known as diabetes is a metabolic disorder which is characterised with increased
blood glucose level. Frequent urination, increased thirst and increased appetite are the common symptoms. Three types
of diabetes are there namely type 1, type 2, and gestational diabetes. Piper longum also known as indian long pepper,
belongs to the family piperaceae. It belongs to the indo-malaya region and tropical rainforest region. Stevia rebaudiana
commonly known as candly leaf, sweet leaf and sugar leaf belongs to the genus stevia. Stevia is a natural sweetener and
natural sugar substitute.
MATERIALS AND METHOD: lpha-amylase inhibition was determined by quantifying the amount of maltose liberated
during the experiment. Different concentration of nanoparticles (10, 20, 30, 40, 50 L.) was pre-incubated with
100 L of amylase solution (1U/mL) at room temperature for 30 minutes. DNSA reagent was added to stop the
reaction and the solution was heated in a water bath for 5 minutes. Control was maintained where the equal
quantity of enzyme extract was replaced by sodium phosphate buffer maintained at a pH value of 6.9.
RESULTS AND DISCUSSION: The bar graph represents the antidiabetic activity of piper longum and stevia herbal formulation
in various concentrations in L. 10 %L of concentration shows 45% of inhibition, 20 % L of concentration shows 50% of
inhibition, 30% of concentration shows 65% of inhibition, 40% of concentration shows 70% of inhibition and 50 %of
concentration shows 80% of inhibition.
CONCLUSION: From this study it is clear that the fruit of piper longum and stevia leaves has proven to be a better choice
for antidiabetic activity.
Key words: Antidiabetic activity, Herbal formulation, Piper longum.
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