The aims of this study were to predict potential activity and interaction conformation of polyphenolic compounds from P. pellucida herbs (14 compounds) with angiotensin-converting enzyme (ACE) macromolecule by in silico molecular docking study. The crystal structure of ACE as a molecular target was obtained from PDB database (PDB ID: 1UZF) with captopril as a native ligand. In silico molecular docking analysis was performed using AutoDockZn (100 docking runs) based on the active site of Zn2+, the central grid was placed on Zn2+ with a box size of 40Á x 40Á x 40Á and a center of 40.835Á x 34.382Á x 44.607Á for selective inhibitors (MCO702) with a spacing of 0.375Á. Based on the docking results demonstrated that the prediction of each polyphenol compounds from P. pellucida has potential of active as ACE inhibitors, it was indicated that docking results of each compound has lower affinity compared to captopril (with binding affinity of -6.36 kcal/mol and the inhibition constant 21.81 μM), where the most moderate binding affinity (the most potential) was tetrahydrofuran lignin ((1R,2S,3S,5R)-3,5-bis(4-hydroxy-3,5-dimethoxyphenyl)cyclopentane-1,2-diyl)bis-(methylene) diacetate) of -8.66 kcal/mol and the highest binding affinity (the less potential) was dillapiole (6-allyl-4,5-dimethoxybenzo[d][1,3]dioxole) of -4.99 kcal/mol, although with different forms of interaction, bond, and constant inhibition. These results are preliminary data for further research with predictions of target compound biological activity and interaction quickly, accurately and inexpensively.
Key words: Angiotensin-converting enzyme, binding affinity, molecular docking, Peperomia pellucida (L) Kunth, polyphenolics
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