This study focuses on determining astaxanthin (AST)s ability to prevent adverse effects of H2 O2 and ultraviolet (UV) irradiation on cells and skin. A Haematococcus pluvialis strain, obtained from Vietnam, was used for AST extraction. It consists of free (4.4% ± 0.7%) and esterified form and accounts for 2.9% ± 0.5% dry weight. 3T3 cells were pre-treated with AST (1, 2.5, 5, 10 µg/ml) or commercial astaxanthin (10 µg/ml) for 24 hours prior to H2 O2 treatment (200 µM, 90 minutes). The results showed that the AST protected 3T3 cells: reduction of mortality rate (16.08%21.52%), senescence-associated β-galactosidasepositive cells (28.9%40.8%), and maintenance of cell proliferation, morphology. AST 5 µg/ml is the optimal concentration in this experiment. Mus musculus var. Albino was treated with a daily dose of topical AST (20 or 200 μg/ml) and UV irradiation for 6 weeks. The results showed that AST reduced wrinkles and retained mouse skins physiology that closed to the control group. AST 20 μg/ml was the best effective concentration in this experiment. In conclusion, AST has been shown to have the ability to protect fibroblasts, skin from the adverse effects of H2 O2 , UV irradiation.
Key words: Astaxanthin, Haematococcus pluvialis, cell senescence, skin aging, hydrogen peroxide, ultraviolet irradiation
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