The present study was an attempt to study alcohol metabolizing and antioxidant properties of Gynostemma pentaphyllum (Thunb.) Makino distillate (GPD), and combination effects with Hovenia dulcis Thunb. extract (HDE) on these activities. The alcohol-metabolizing activity of GPD with/without HDE was evaluated by assessing alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities. To define the effect of GPD with/without HDE on alcohol metabolism, antioxidant activities and total phenolic content of GPD with/without HD extract were evaluated using 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferrous chelating assays and the Folin-Ciocalteu method. Cytotoxicity against human normal liver CHANG cells was also evaluated using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. GPD treatment alone or in combination with HDE significantly increased ADH and ALDH activities; combined treatment was most effective. The contents of total phenolic and flavonoid were greater in combination than the level found in GPD alone. GPD revealed synergistic antioxidant effect when combined with HDE. GPD and/or HDE had no antiproliferative activity against the normal liver cell line. These results suggest that GPD-HDE combination is the potential natural resource for the management of ethanol-induced liver toxicity.
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