Spent oyster mushroom (Pleurotus florida) compost as tremendous source for isolation of industrial significant enzymes such as Amylase, Protease and Cellulase. This study was conducted in order to achieve efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), Cellulase (EC 3.2.1.4) and Protease (EC 3.4.21.14) from spent oyster mushroom (Pleurotus florida) compost waste. Optimal enzyme recovery was achieved when spent oyster mushroom compost wastes and concentrated by acetone precipitation. The purification was performed by column chromatography. The enzymes such as cellulase, amylase and protease released from oyster mushroom (Pleurotus florida) compost waste were showed activities of 15.78 U/ml, 3.42 U/ml and 0.042 U/ml respectively. These were utilized in various industrial and environmental applications such as Starch processing in potato waste from food industry using amylase and Biotreatment of cotton waste using cellulase.
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