Objective: To identify the role of peroxisome proliferator-activated receptor ( PPAR)-α gene in Dyslipidemia.
Methodology: This experimental study was carried out at Center for research in experimental and applied medicine (CREAM) Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi from December 20, 2016 to June 8, 2017. The study comprised of three groups; Group one included subjects who were healthy, group two was comprised of diabetic without dyslipidemia and group three included diabetics with dyslipidemia. Blood was collected and Primers specific to PPAR-α were designed on the basis of sequence available on website Primer BLAST. RNA was extracted with in first six hours of sample collection using Gene JET RNA purification kit (Thermo Scientific). For first strand cDNA synthesis Revert Aid Premium was utilized. Polymerase chain reaction was used for the isolation, amplification or detection of specific nucleotide sequences in genomic DNA. PCR products were visualized by Agarose gel electrophoresis. After Real time PCR, molecular analysis was quantified by comparative CT Method (ΔΔ Ct method). Positive value means down regulation and negative value means upregulation.
Results: Primers were optimized using conventional PCR. A single band at 224 base pair represented the amplification of PPAR-α gene. The PCR protocol was optimized for PPAR-α gene.
Conclusion: PPAR-α gene has important role in the homeostasis of lipid profile in the body. Upregulation or increased expression of this gene leads to reduction in lipid profile, while down regulation or suppression of this gene leads to deranged lipid levels.
Key words: Dyslipidemia, gene expression, metabolic process.
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