In the present study sodium carbonate peroxyhydrate (2Na2CO3.3H2O2) which is considered as eligible algaecide and fungicide, was used as an alternative source of oxygen in fish ponds with concentration of 50 and 100 mg/L for 10 days. To approach our target hematological (RBCs, Hb,PCV, total and differential leukocytic count) together with several serum biochemical parameters as (glucose ,total proteins ,albumin, globulins, A/G, amino transferases (ALT,AST),urea and creatinine) were estimated. DNA fragmentation and a mild degree of DNA degradation as shown by DNA smear and a hallmark apoptosis were observed in samples from gills, brain and liver after 6 days of the experiment. Pathological changes started to appear first in the brain tissue then other organs (liver and kidney) were also affected after 6 days of the experiment with 100mg/L treated groups indicating the sensitivity of the brain and the direct effect of hypoxia on this organ. Thus, sodium carbonate peroxyhydrate (OXY-SOS) can be used as a stable oxygen releaser and as a suitable alternative to artificial aeration in fish farms for a period up to 6 days without adverse effect on treated fish.
Key words: Oxygen, Nile Tilapia, Sodium carbonate peroxyhydrate, DNA fragmentation
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