The field of ornamental plant genomics has increased the sequencing of the whole genome of ornamental plants in the past 10 years. Phoenix roebelenii (pygmy date palm) is a popular ornamental plant grown indoors and outdoors. Pygmy date palm is a tropical and subtropical plant that belongs to the family Arecaceae. This plant is resistant to pests, tolerant to soil variation, and tolerant to drought. Therefore, it is interesting to report the nuclear and organelle genome sequences of P. roebelenii. The raw genome data were retrieved from NCBI and cleaned with AdapterRemoval version 2.3.2 for high-quality clean data. The genome size was estimated using KmerGenie version 1.7051, and nuclear genome assembly was generated using MaSurCa version 3.3.2. The completeness and quality of genome assembly is assessed using BUSCO version 4.1.2. This analysis resulted in a draft nuclear genome sequence constituting 462,152,837 bps with 7019 scaffolds. The repeats, genes, tRNA genes, and transcription factors were identified and predicted using RepeatModeler version 2.0.1, AUGUSTUS version 3.3.2, tRNAscan-SE version 2.0.6, and plant TFDB version 4.0, respectively. In total, 35.11% of repeats, 42,388 genes, 480 tRNA genes, and transcription factors for 480 genes were predicted. The functional annotation was based on UniProt protein database, OrthoFinder version 2.2.7, InterproScan, Plant metabolic network (PMN) analysis, and gene ontology (GO) categorization. The organelle genome sequences – chloroplast genome sequence and mitochondrial genome sequence are reported in the study. The mitochondria and chloroplast assembly were generated using GetOrganelle version 1.6.4. The chloroplast genome consists of 125,222 bps, and the mitochondrial genome consists of 482,735 bps. The chloroplast and mitochondrial genome annotation was performed using CPGAVAS2 version 1 and AGORA version 1, respectively. The chloroplast genome has 108 genes, 30 tRNA genes, and 136 repeats, whereas the mitochondrial genome has 65 genes, 12 tRNA genes, and 91 repeats. Thus, this study reports the draft nuclear and complete organelle genome sequences of P. roebelenii.
Key words: Chloroplast genome, Genome assembly, Mitochondrial genome, Nuclear genome, Phoenix roebelenii, Pygmy date palm
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