A total of 16 isolates of lactic acid bacteria (LAB) from flowers were screened for the bile salt hydrolase activity (BSH) on MRS agar supplemented with 0.5% (w/v) taurodeoxycholic acid (TDCA). The isolates were divided into two group based on their phenotypic characteristics and 16S rRNA gene sequences of the representative isolates were determined. Group I isolates were the members of genus Enterococcus. Isolates FM1-1, FM1-2, FM12-1 and FM12-2 were identified as Enterococcus durans (100% similarity), isolate FM2-3 was identified as Enterococcus gallinarum (99.92% similarity) while the isolate FM11-2 was identified as Enterococcus lactis (99.77 % similarity). Group II isolates were the members of genus Lactobacillus and they were identified as Lactobacillus plantarum subsp. plantarum (the representative isolates, FM3-1 and FM16-2 showed 100% similarity). Eleven isolates including FM1-1, FM1-2, FM2-3, FM3-1, FM4-2, FM11-2, FM12-1, FM12-2, FM14-1, FM14-2, and FM16-2 exhibited bile salt hydrolase activity. All LAB isolates showed the cholesterol assimilated ability ranged from 9.57 to 51.69%. The isolate FM11-2 efficiently assimilated the cholesterol with 51.69%.
Key words: bile salt hydrolase, cholesterol assimilation, flower, lactic acid bacteria, probiotic
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