Most dairy cows suffered negative energy balance in early lactation that can affect their fertility .Adding fat to the cattle diet are used to increase the diet energy content . In this study level of ketone bodies (acetone and acetoacetate ) , nonestratefied fatty acids( NEFAs) and some individual fatty acids in serum and milk used as biomarkers for negative energy balance and fertility in dairy cows supplemented with two fat types. A comparison of calcium salts of palm oil fatty acid versus MixPromega-3 diet on the level of fatty acids in serum and milk of early lactating Holstein cows was valued and their relation to fertility. The diets were formulated to be isonitrogenous and isocaloric .The two diets given from day 0 to 30 days postpartum. Samples of blood and milk were collected at 1st,2nd and3rd weeks after calving. Results revealed that the increased serum and milk linoleic and linolineic acid with decreased saturated (palmitic and stearic ) fatty acids and oleic acid are related to improved energy status with decreasing the ketone bodies and nonestratefied fatty acids and enhance the fertility of dairy cattle fed Promixomega-3 when compared with calcium soap fed group . .Level of ketone bodies and nonestratefied fatty acids increased in milk than in serum in the same group. The fertility was enhanced by giving Promix omega -3 diet compared with calcium soap diet.
Key words: Dairy cattle, fatty acids, Fat supplement, ketone bodies
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