Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

J App Pharm Sci. 2021; 11(8): 139-146

Microbial profiling of wound pathogens in isolates from an Egyptian hospital using a microarray chip

Mohamed Mohamed Adel El-Sokkary.

Chronic polymicrobial infections represent diagnostic challenges for both molecular and culture methods. Chronic wounds, inducing chronic pain and reducing the mobility of individuals, have a notable effect on the quality of life. At any given time, 1% of the population is usually affected and, therefore, multiple treatments are also required. Identification of the etiology of such infections facilitates the appropriate treatment. In this study, 20 wound samples were collected from 20 patients with suspected wound infection. The panel of the current assay targets 12 clinically relevant aerobic pathogens, commonly associated with chronic wound infection. Microbial wound infections were detected by both usual polymerase chain reaction (PCR) and subsequently testing using the DNA chip. In the current study, the results of culture-free bacterial identification using the two methods of DNA analyses were compared. By molecular detection using PCR, seven different bacterial species were identified: Citrobacter spp. (100%), Enterobacter spp. (100%), Klebsiella pneumoniae (100%), Pseudomonas aeruginosa (90%), Proteus spp. (80%), Escherichia coli (60%), and Staphylococcus aureus (10%). Mixed microbial infections were detected in all samples indicating four, five, or six different bacteria, identified in each sample. Microarray detection in comparison to PCR indicated 100% matching. These results demonstrate the possibility of fast identification of wound infection pathogens even in a mixed culture in a very short time, which in turn facilitates the proceeding steps for proper treatment.

Key words: Wound infection-Mixed infection-Microbial diversity-PCR-Microarrays

Similar Articles

Insufficient ventilation led to a probable long-range airborne transmission of SARS-CoV-2 on two buses.
Ou C, Hu S, Luo K, Yang H, Hang J, Cheng P, Hai Z, Xiao S, Qian H, Xiao S, Jing X, Xie Z, Ling H, Liu L, Gao L, Deng Q, Cowling BJ, Li Y
Building and environment. 2022; 207(): 108414

Clinical Utility of Methylation-Specific Multiplex Ligation-Dependent Probe Amplification for the Diagnosis of Prader-Willi Syndrome and Angelman Syndrome.
Kim B, Park Y, Cho SI, Kim MJ, Chae JH, Kim JY, Seong MW, Park SS
Annals of laboratory medicine. 2022; 42(1): 79-88

Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.
Taracena ML, Garcia Caffaro I, Paiva-Silva GO, Oliveira PL, Rendon PA, Dotson EM, Pennington PM
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 279-294

Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation.
Kaplanoglu E, Kolotilin I, Menassa R, Donly C
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 235-252

Contrast-Based and Arm-Based Models for Network Meta-Analysis.
Karahalios A, McKenzie JE, White IR
Methods in molecular biology (Clifton, N.J.). 2022; 2345(): 203-221

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.

ScopeMed Web Sites