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Proliferative effects of glial cell-derived neurotrophic factor on putative spermatogonial stem cells derived from Zebu crossbred (Bos indicus X Bos taurus) malesSunil Kumar, Arumugam Kumaresan, Kaustubh K. Saraf, Subhash Gahalot, Utkarsh Kumar Tripathi, Tushar Kumar Mohanty. Abstract |  Download PDF | |  Post | Background:
Reports on the effects of glial cell-derived neurotrophic factor (GDNF) on spermatogonial stem cells (SSCs) of Zebu or crossbred bovine are lacking. Proliferative effects were reported in ovine and caprine, whereas non-proliferative effects were only observed in Bos taurus.
Aim:
Understanding the roles of GDNF in maintaining germ cell proliferation may be crucial for reducing the high rate of reproductive failure in crossbred (Bos indicus X Bos taurus) bovine.
Methods:
In the present study, the in vitro culture effects of GDNF on SSCsof crossbred bovine were assessed. In the treatment groups, SSCs were cultured in a medium supplemented with two different doses of GDNF (T1-10ng/mL, T2-40ng/mL) and co-cultured with the Sertoli cell layer (T3). In the control group, SSCs were grown without GDNF or without co-culture (C). The proliferative effects in terms of the number of cells and colonies were determined on days 4, 7, 10, and 13 of in vitro culture.
Results:
There was a significant (p
Key words: Bovine; GDNF; Sertoli cells; Spermatogonial stem cells.
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