Abstract
Our study was carried out to determine the phenotypic characterization and antibiogram of Plesiomonas shigelloides from fresh water fishes in the Federal Capital Territory (FCT), Nigeria. P. shigelloidesis an aquatic microorganism has been implicated in outbreaks of food poisoning and is coming to be commonly regarded as a cause of gastroenteritis in humans. The method used was cultural isolation, selective plating, Preliminary identification, biochemical identification and antibiogram. The biochemical characterization was carried out using oxidase, catalase, methyl red, voges Proskauer, Triple sugar iron, urease, citrate utilization test and indole test. Confirmation of the isolates was done using MicrobactTM GNB24E identification kit (Oxoid, UK). Total of 400 fishes (301 Clarias gariepinus and 99 Oreochromis niloticus) were bought from fresh water ponds and fish selling market from three Area Councils in the FCT in 2023. An overall prevalence rate of 4(1%) of P. shigelloides isolates was recorded ands Area Council, Gwagwalada had the highest prevalence rate of 2(1.49%) while Kuje and AMAC had (1) 0.75% each. The susceptibility of P. shigelloides was; Ofloxacin (100%), Gentamicin, Neticillin and Tetracycline (75%), Levofloxacin (50%) while Ceftriaxone and Co-trimoxaxole was (25%) while the resistance was Amoxiclav (100%), Cotrimoxaxole, Tetracycline, Ceftazidine and Erythromycin (75%) while Neticillin was (25%). The Multiple Antibiotic Resistance Index and Multiple Drug Resistance from this study was 4 (100%) indicating a high level of drug resistance. Our research showed that P. shigelloides isolates from fishes in FCT could pose potential threat to human population and dissipated resistance to commonly used antimicrobial agents.
Key words: Key words: Plesiomonas shigelloides, Biochemical, MicrobactTM GNB24E, Antibiotic Susceptibility, Multiple Antibiotic index, Multiple Drug Resistance.
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