Alzheimers disease (AD) is a progressive neurodegenerative disease of the central nervous system. Inflammatory injury and oxidative stress have been a hallmark for AD due to free radical production. The main objective of the present study is to identify the compounds having good antioxidant properties from Lactobacillus plantarum and its effect on neuronal cells. L. plantarum strain was cultured in De Man, Rogosa and Sharpe (MRS) medium supplemented with cinnamon and pantothenic acid (40 μg/ml and 4 μg/ml). Solvent extraction (ethyl acetate, chloroform, petroleum ether, and hexane) was done in the supernatant and tested for in vitro 2,2-diphenyl-1-2- picrylhydrazyl (DPPH), H2O2 scavenging activity, and H2O2-induced cytotoxicity assessment in PC12 cell lines. The results obtained from the study showed that all the extracts have good antioxidant activity. It was observed that the stress-induced PC12 cell lines showed maximum protection of cells in the Media D (chloroform) extract, the IC50 value was recorded as 23.71 μg/ml. Similarly, all solvent extracts showed significant antioxidant activity in DPPH assay with IC50 value ranging from 23.59 to 106.8 μg/ml and IC50 value in H2O2 scavenging assay ranging from 37.39 to 107.7 μg/ml. Referring to the complex multifactorial etiology of AD, the findings from our work exhibited remarkable potentials of antioxidants activity.
Key words: Alzheimers disease, Lactobacillus plantarum, DPPH scavenging assay, H2O2 scavenging assay, PC12 cell line
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