The mycelial biomass production of four strains (WS218-1, WS218-2, CST01, CST02) of Pleurotus cystidiosus in liquid culture using coconut water as medium was evaluated. Mycelia were extracted and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and total phenolic contents were analyzed. The mycochemicals of the best strain were also screened using thin layer chromatography (TLC) spot test. Results revealed that WS218-2 strain produced the heaviest mycelial biomass (6.57 g fresh wt. and 0.39 g dry wt.) while CSC02 and WS218-1 strains registered the lowest fresh and dry weight, respectively. Ethanolic extract of WS218-2 strain showed the highest radical scavenging activity (79.01%) and contained the highest phenolic content (95.67 mg GAE / g of sample). Eleven mycochemicals including essential oil, triterpenes, anthraquinones, tannins, flavonoids, phenols, fatty acids, alkaloids, steroids, sugars, and coumarins were detected in WS218-2 mycelia. P. cystidiosus mycelia could be valuable source of natural antioxidants and bioactive metabolites and it is strain dependent.
Key words: Wild strains, strain dependent, mycochemicals, antioxidant, WS218-2, P. cystidiosus
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